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KMID : 0370219980420030248
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Yakhak Hoeji
1998 Volume.42 No. 3 p.248 ~ p.256
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Synthesis and Biological Activity of 5-S-GAD ( N-β-alanyl-5-S-glutathionyl-3 , 4-dihydroxyphenylalanine ) , a Novel Antibacterial Substance
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임재윤/Leem JY
박호용/Natori Shunji/Park HY/Natori SJ/
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Abstract
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We had already reported that we purified N-beta-alanyl-5-S-glutathionyl-3,4-dihydroxyphenylalanine (5-S-GAD), a novel antibacterial substance from the immunized adult Sarcoph aga peregrina (Flesh fly). We found that the antibacterial activity of synthetic 5-S-GAD is equal to that of authentic 5-S-GAD without a specificity of antibacterial activity against Gram positive and Gram negative. Significant synergism was detected between 5-S-GAD and streptomycin against streptomycin resistant strain E.coli K12 594. It has an antitumor activity against several tumor cell lines at a concentration of 100mcM. However, no cytotoxic activity against murine macrophage was detected at a concentration of 500mcM. Furthermore, haemolytic activity against sheep erythrocytes was not detected at the same concentration. We suggest that the S-conjugation of glutathion with dihydroxyphenylalanine might be important to increase antibacterial activity of dihydroxyphenylalanme.
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